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Results The newly obtained ITS2 sequences of Ankyra judayi, Atractomorpha porcata and Sphaeroplea annulina of the ' Sphaeroplea ' clade do not show any branching in the secondary structure of their helix I.

Alexander Keller et al.

BMC Evolutionary Biology 8 (7), 25 Jul 2008

Methods DNA extraction, amplification and sequencing Extraction of genomic DNA from cultured cells of Ankyra judayi, Atractomorpha porcata and Sphaeroplea annulina was done using Dynabeads ® (DNA DIRECT Universal, Dynal Biotech, Oslo, Norway) according to the manufacturer's protocol.

Alexander Keller et al.

BMC Evolutionary Biology 8 (7), 25 Jul 2008

The ITS2 secondary structure of Atractomorpha porcata was used as template for homology modelling.

Alexander Keller et al.

BMC Evolutionary Biology 8 (7), 25 Jul 2008

Smith) Fott 1957 SAG 17.84 EU352800 Atractomorpha porcata Hoffman 1984 strain SAG 71.90 EU352803 Sphaeroplea annulina (Roth) C.

Alexander Keller et al.

BMC Evolutionary Biology 8 (7), 25 Jul 2008

Using three newly obtained ITS2 sequences from Ankyra judayi , Atractomorpha porcata and Sphaeroplea annulina (Sphaeropleaceae) in this study we aimed to pursue two consecutive questions concerning the phylogenetic relationships within Chlorophyceae. (1) What is the phylogenetic position of the newly sequenced algae relative to the 'core Sphaeropleales' and could the biflagellate DO-group be regarded as monophyletic? (2) How does the secondary structure of the new ITS2 sequences look like and is an autapomorphic feature of the secondary structure associated with the monophyletic DO-group?

Alexander Keller et al.

BMC Evolutionary Biology 8 (7), 25 Jul 2008

 
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