dict.md logo
Choose languages of interest
SELECT >>
English
Advertisement:
Translation
 
0 translations
 
Definition
 
enEnglish
Binding Sites definition: The parts of a macromolecule that directly participate in its specific combination with another molecule.
binding site definition: reactive portion of a macromolecule that directly participates in its specific combination with another molecule.
Binding Sites, Antibody definition: Local surface sites on antibodies which react with antigen determinant sites on antigens. They are formed from parts of the variable regions of FAB FRAGMENTS.
Antibody Binding Site definition: The site at which an antibody makes direct physical contact with a corresponding epitope on an antigen.
 
Examples
 

It was revealed that d(T[ c , s ]T) binds to the central part of the antibody-combining site, while the CPD-flanking nucleotides bind to the positively charged area of the V H domain via electrostatic interactions.

Takuya Torizawa et al.

Nucleic Acids Research 28 (4), 15 Feb 2000

Although the model might simply represent one of the possible multiple conformations at most and not consider any conformational change upon binding to d(T[ c , s ]T), it could at least be concluded that d(T[ c , s ]T) binds the central parts of the antibody-combining site.

Takuya Torizawa et al.

Nucleic Acids Research 28 (4), 15 Feb 2000

Although the 31 P NMR data suggest the existence of a conformational exchange of the CPD-flanking nucleotides on the protein surface ( vide supra ), the residues that exhibit line broadening upon binding to each of the spin-labeled antigens are clustered in a specific area in the V H domain, indicating that the orientations of CPD-containing nucleotides are fairly fixed in the antibody-combining site: both the 3′- and 5′-sides of the flanking deoxynucleotides orient towards the V H domain, as predicted from the chemical shift perturbation data.

Takuya Torizawa et al.

Nucleic Acids Research 28 (4), 15 Feb 2000

On the basis of these data, along with the fact that d(T[ c , s ]T) binds the central parts of the antibody-combining site, we concluded that CPD-containing oligonucleotides bind TDM-2 in a crooked form (a boomerang shape), draping the 3′-side nucleotides onto the H1 and H3 segments and the 5′-side nucleotides onto the H2 and L3 segments.

Takuya Torizawa et al.

Nucleic Acids Research 28 (4), 15 Feb 2000

Tel: +1 847 491 7849; Fax: +1 847 491 4928; Email: t-wu@nwu.edu 1 1 2000 28 1 214 218 23 7 1999 13 10 1999 13 10 1999 Copyright © 2000 Oxford University Press 2000 The Kabat Database was initially started in 1970 to determine the combining site of antibodies based on the available amino acid sequences at that time.

George Johnson et al.

Nucleic Acids Research 28 (1), 01 Jan 2000

1 further example >>
Publications
 

Reactive site polymorphism in the murine protease inhibitor gene family is delineated using a modification of the PCR reaction (PCR + 1).

F Borriello et al.

Nucleic Acids Research , 25 Sep 1990

Reactivity of B and Z-DNA towards N-acetoxy-2-acetylaminofluorene.: ... radioactivity. Covalent binding of carcinogen to the polymers was evaluated after separation of the reacted polymers from non-reacted carcinogen by thin-layer chromatography. We found that B-form polymer reacts twice faster than the Z-form polymers. Proportions of main adducts in the three polymers are almost the same. Results are discussed in relation to the calculated electrostatic potential minima and steric accessibility at the reactive site (1, 2).

M Spodheim-Maurizot et al.

Nucleic Acids Research , 24 Jul 1982

High resolution footprinting of a type I methyltransferase reveals a large structural distortion within the DNA recognition site.: ... is a remarkably hyper-reactive site on each strand. The two ...

D R Mernagh et al.

Nucleic Acids Research , 15 Dec 1996

Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases: ... determined by its reactive site (Arg346-Met347), which ...

Qingcai Wang et al.

BMC Biochemistry , 08 Jul 2003

Tandem duplication, circular permutation, molecular adaptation: how Solanaceae resist pests via inhibitors: ... amino acid sites: the reactive site is under positive ...

Lesheng Kong et al.

BMC Bioinformatics , 13 Feb 2008

Isolation, Cloning and Structural Characterisation of Boophilin, a Multifunctional Kunitz-Type Proteinase Inhibitor from the Cattle Tick: ... reveals a non-canonical binding mode to the proteinase. The N-terminal region of the mature inhibitor, Q16-R17-N18, binds in a parallel manner across the active site of the proteinase, with ...

Sandra Macedo-Ribeiro et al.

PLoS ONE , 20 Feb 2008