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Lactobacillus brevis definition: A species of gram-positive, rod-shaped LACTIC ACID bacteria that is frequently used as starter culture in SILAGE fermentation, sourdough, and lactic-acid-fermented types of beer and wine.
 
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Lactobacillus brevis ATCC 8287 is covered by a regular surface (S-) layer consisting of a 435 amino acid protein SlpA.

Silja Åvall-Jääskeläinen et al.

BMC Microbiology 8 (7), 01 Oct 2008

Conserved carbohydrate binding motifs were identified in the positively charged N-terminal regions of six Lactobacillus brevis S-layer proteins.

Silja Åvall-Jääskeläinen et al.

BMC Microbiology 8 (7), 01 Oct 2008

The S-layer protein of Lactobacillus brevis ATCC 8287, SlpA [ 16 ], is a 435 amino acid, 46 kDa protein, which assembles on the bacterial cell forming an oblique lattice [ 17 ] and for which a fibronectin-binding function has been described [ 18 ].

Silja Åvall-Jääskeläinen et al.

BMC Microbiology 8 (7), 01 Oct 2008

Lactobacillus brevis ATCC 8287 and Lactobacillus acidophilus ATCC 4356 were grown in MRS (Difco, Detroit, MI, USA) at 37°C.

Silja Åvall-Jääskeläinen et al.

BMC Microbiology 8 (7), 01 Oct 2008

Strains and plasmids used in this study Strain or plasmid Relevant properties a Reference or source Strains Lactobacillus brevis ATCC 8287 ATCC Lactobacillus acidophilus ATCC 4356 ATCC Escherichia coli DH5αF' F' endA1 hsd17 (r k - m k + ) supE44 thi-1 recA1 gyrA (NaI r ) relA1Δ (lacIZYA-argF) U169 deoR [ϕ80 d lacΔ(lacZ) M15] 58 Escherichia coli BL21(DE3) F - ompT hsdS B (r B - m B - ) gal dcm (DE3) Novagen Plasmids pET-28a(+) Km r , E. coli expression vector Novagen pET-28b(+) Km r , E. coli expression vector Novagen pKTH5198 Km r , pET-28b(+)( Nco I/ Xho I::SlpA 1–435 -linker thrombin -Tag his 6 ) This study pKTH5199 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 1–435 ) This study pKTH5200 Km r , pET-28b(+)( Nco I/ Xho I::SlpA 146–435 -linker thrombin -Tag his 6 ) This study pKTH5201 Km r , pET-28b(+)( Nco I/ Xho I::SlpA 291–435 -linker thrombin -Tag his 6 ) This study pKTH5203 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 1–145 ) This study pKTH5204 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 1–290 ) This study pKTH5258 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 190–423 ) This study pKTH5259 Km r , pET-28a(+)( NheI ::Tag his 6 -linker thrombin -SlpA 210–423 ) This study pKTH5260 Km r , pET-28a(+)( NheI ::Tag his 6 -linker thrombin -SlpA 1–189 ) This study pKTH5261 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 190–435 ) This study pKTH5262 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 210–435 ) This study pKTH5264 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 167–435 ) This study pKTH5325 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 179–435 ) This study pKTH5333 Km r , pET-28a(+)( Nhe I::Tag his 6 -linker thrombin -SlpA 149–435 ) This study a Km r , resistance to kanamycin

Silja Åvall-Jääskeläinen et al.

BMC Microbiology 8 (7), 01 Oct 2008

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