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Translation
 
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4 words
Antibodies, Monoclonal, Flow Cytometry Antikörper, monoklonal, Flow-Zytometrie
 
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enEnglish frFrench
1 word
cytometry cytométrie
 
Definition
 
enEnglish
Flow Cytometry definition: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
flow cytometry definition: technique for sorting, selecting or counting individual cells in a suspension as they pass through a tube, especially techniques involving the detection of a cell bound fluorescent label; other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
Flow Cytometry definition: A technique for counting, examining and sorting microscopic particles suspended in a stream of fluid.
Flow cytometry (FC) definition: Flow Cytometry
Reagents, Cytology/Histology, Flow Cytometry definition: Reagents used in cytologic assays performed by flow cytometers. They include labels for cell membranes and standards to normalize instrument setup, cell count, and proliferation. These reagents are frequently available in kits that allow the calibration, control, and standardization of flow cytometry analysis.
Image Cytometry definition: A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.
Cytometry definition: The measurement of physical and /or chemical characteristics of cells, or, by extension, of other biological particles.
Reagents, Cytology/Histology, Calibration, Flow Cytometry definition: Cytology/histology reagents intended to establish points of reference (i.e., calibrate) for assays using flow cytometry techniques. These reagents typically consist of fluorescent labeled latex microspheres; they are usually available as calibration kits.
Reagents, Cytology/Histology, Control, Flow Cytometry definition: Cytology/histology reagents intended for quality control for assays using flow cytometry techniques. These reagents typically consist of fluorescent labeled monoclonal antibodies of known values; they are usually available as control kits.
Laser Scanning Cytometry definition: A scanning microscope-based, cytofluorimetry technique for making fluorescence measurements and topographic analysis on individual cells. Lasers are used to excite fluorochromes in labeled cellular specimens. Fluorescence is detected in multiple discrete wavelengths and the locational data is processed to quantitatively assess APOPTOSIS; PLOIDIES; cell proliferation; GENE EXPRESSION; PROTEIN TRANSPORT; and other cellular processes.
4 further definitions >>
frFrench
cytométrie definition: Méthode permettant de mesurer certains paramètres des cellules.
 
Examples
 

Background Cytokine flow cytometry (CFC) or intracellular cytokine staining (ICS) can quantitate antigen-specific T cell responses in settings such as experimental vaccination.

Holden T Maecker et al.

BMC Immunology 6 (7), 24 Jun 2005

Background Enzyme-linked immunospot (ELISPOT) and cytokine flow cytometry (CFC) (or more specifically, intracellular cytokine staining (ICS)) are popular methods for single-cell analysis of antigen-specific T cell cytokine production.

Holden T Maecker et al.

BMC Immunology 6 (7), 24 Jun 2005

Of the two types of assays, ICS has the advantage of a highly multiparametric read-out (flow cytometry) that allows for precise phenotyping of the responding T cell populations.

Holden T Maecker et al.

BMC Immunology 6 (7), 24 Jun 2005

Instrumentation and setup The flow cytometry instrumentation used in this study included 12 BD FACS Caliburs (BD Biosciences), 3 BD LSRIIs (BD Biosciences), and 1 CyAn (Dako Cytomation, Fort Collins, CO).

Holden T Maecker et al.

BMC Immunology 6 (7), 24 Jun 2005

Suni MA Dunn HS Orr PL deLaat R Sinclair E Ghanekar SA Bredt BM Dunne JF Maino VC Maecker HT Performance of plate-based cytokine flow cytometry with automated data analysis BMC Immunology 2003 4 9 12952557 Maecker HT Hawley TS, Hawley RG Cytokine flow cytometry Flow Cytometry Protocols Meth Molec Biol Walker J 2004 2nd Totowa, NJ , Humana Press 95 107 Cox JH Ferrari G Kalams SA Lopaczynski W Oden N Group ELISPOTS Results of an ELISPOT proficiency panel conducted in 11 laboratories participating in international human immunodeficiency virus type 1 vaccine trials.

Holden T Maecker et al.

BMC Immunology 6 (7), 24 Jun 2005

643 further examples >>
Publications
 

Standardization of cytokine flow cytometry assays

Holden T Maecker et al.

BMC Immunology , 24 Jun 2005

Sensitive detection of RNAs in single cells by flow cytometry.

H Yu et al.

Nucleic Acids Research , 11 Jan 1992

Flow : Statistics, visualization and informatics for flow cytometry

Jacob Frelinger et al.

Source Code for Biology and Medicine , 17 Jun 2008

Use of a high affinity DNA ligand in flow cytometry.

K A Davis et al.

Nucleic Acids Research , 15 Feb 1996

Impact of cryopreservation on tetramer, cytokine flow cytometry, and ELISPOT

Holden T Maecker et al.

BMC Immunology , 18 Jul 2005

Flow Cytometry of Human Primary Epidermal and Follicular Keratinocytes

Alfredo Gragnani et al.

Eplasty , 19 Feb 2008

Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma

Qin Huang et al.

BMC Clinical Pathology , 30 May 2008

A perspective for biomedical data integration: Design of databases for flow cytometry

John Drakos et al.

BMC Bioinformatics , 14 Feb 2008

Performance of plate-based cytokine flow cytometry with automated data analysis

Maria A Suni et al.

BMC Immunology , 02 Sep 2003

Study of membrane potential in T lymphocytes subpopulations using flow cytometry

Fernanda Mello de Queiroz et al.

BMC Immunology , 03 Nov 2008

638 further publications >>