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Blotting, Southern definition: A method (first developed by E.M. Southern) for detection of DNA that has been electrophoretically separated and immobilized by blotting on nitrocellulose or other type of paper or nylon membrane followed by hybridization with labeled NUCLEIC ACID PROBES.
southern blotting definition: method of identifying DNA by cDNA or RNA hybridization after electrophoresis and transfer to nitrocellulose or other fixative.
Southern Blotting definition: A technique for the detection of specific DNA fragments that have been separated by polyacrylamide-gel electrophoresis, transferred to a nitrocellulose or other type of paper or nylon membrane, and detected by hybridization and visualization with a labeled probe.
Southern Blot definition: Molecular, Southern Blot
DNA Probes definition: Species- or subspecies-specific DNA (including COMPLEMENTARY DNA; conserved genes, whole chromosomes, or whole genomes) used in hybridization studies in order to identify microorganisms, to measure DNA-DNA homologies, to group subspecies, etc. The DNA probe hybridizes with a specific mRNA, if present. Conventional techniques used for testing for the hybridization product include dot blot assays, Southern blot assays, and DNA:RNA hybrid-specific antibody tests. Conventional labels for the DNA probe include the radioisotope labels 32P and 125I and the chemical label biotin. The use of DNA probes provides a specific, sensitive, rapid, and inexpensive replacement for cell culture techniques for diagnosing infections.
Reagents, Molecular Assay, Nucleic Acid Probe, DNA definition: Molecular assay reagents consising of a nucleic acid probe with a DNA chemical structure. DNA probes may be radioactively or nonradioactively (both enzymatic and nonenzymatic) labeled. DNA probes are used in tests to obtain information about the genetic characteristics of a sample; these procedures are generically known as nucleic acid assays and include hybridization assays, amplification methods (e.g., polymerase chain reaction), and electrophoretic separation.
DNA Probes definition: A piece of DNA that has been labeled, usually radioactively or with a fluorescent dye, which is used in hybridization studies. Applications include Northern and Southern blots, in situ hybridization techniques, and diagnostic tests. DNA probes can be highly specific or degenerate.
Hybridization, Genetic definition: The genetic process of crossbreeding between genetically dissimilar parents to produce a hybrid.
Nucleic Acid Hybridization definition: Widely used technique which exploits the ability of complementary sequences in single-stranded DNAs or RNAs to pair with each other to form a double helix. Hybridization can take place between two complimentary DNA sequences, between a single-stranded DNA and a complementary RNA, or between two RNA sequences. The technique is used to detect and isolate specific sequences, measure homology, or define other characteristics of one or both strands. (Kendrew, Encyclopedia of Molecular Biology, 1994, p503)
nucleic acid hybridization definition: "zipping" reaction between complementary DNA or RNA single strands to form a duplex (double stranded) molecule; basis of many techniques for assaying nucleic acid sequence complementarity.
22 further definitions >>

The kinetics of in situ hybridization.

P Szabo et al.

Nucleic Acids Research , 01 May 1975

Hybridization properties of immobilized nucleic acids.

T R Gingeras et al.

Nucleic Acids Research , 10 Jul 1987

A colorimetric method for DNA hybridization.

M Renz et al.

Nucleic Acids Research , 25 Apr 1984

In situ hybridization with fluoresceinated DNA.

J Wiegant et al.

Nucleic Acids Research , 25 Jun 1991

A kinetic model for subtractive hybridization.

J J Milner et al.

Nucleic Acids Research , 11 Jan 1995

A simulation of subtractive hybridization.

T J Cho et al.

Nucleic Acids Research , 15 Mar 1998

Reverse Southern hybridization.

H B Gamper et al.

Nucleic Acids Research , 22 Dec 1986

A Mathematical Model for Suppression Subtractive Hybridization

Chetan Gadgil et al.

Comparative and Functional Genomics , 01 Oct 2002

Hybridization kinetics and thermodynamics of molecular beacons

Andrew Tsourkas et al.

Nucleic Acids Research , 15 Feb 2003

Universal bases for hybridization, replication and chain termination

Markus Berger et al.

Nucleic Acids Research , 01 Aug 2000

2472 further publications >>