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impaled aufgespießt
impaled gepfählt
 
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Abstract Simultaneous electrophysiology and confocal microscopy were used to investigate purinergic neurotransmission at single smooth muscle cells (SMCs) in mouse isolated vas deferens, and to explore the relationship between two high-resolution P2X-receptor-mediated measures of per pulse ATP release: transient peaks in the first time derivative of the rising phase of excitatory junction potentials (EJPs) recorded in single SMCs (‘discrete events’; DEs) and neuroeffector Ca 2+ transients (NCTs) in the impaled SMCs.

J.S. Young et al.

Neuroscience 148 (1), 10 Aug 2007

This study shows that discrete events represent neurotransmitter release onto the impaled cell.

J.S. Young et al.

Neuroscience 148 (1), 10 Aug 2007

First, the median amplitude of the first derivative of the EJP was larger when there was a coincident NCT in the impaled cell, compared with instances when no coincident NCT occurred.

J.S. Young et al.

Neuroscience 148 (1), 10 Aug 2007

Second, the time-to-peak amplitude of the first derivative was shorter if there was a coincident NCT in the impaled cell, compared with when no coincident NCT was observed within the field.

J.S. Young et al.

Neuroscience 148 (1), 10 Aug 2007

More likely is that every sEJP was triggered by release of a single packet of ATP directly onto the impaled SMC and that the amplitude variation was due either to variability in the size of the released ATP packets, or in the responses of P2X-receptors on the SMC surface ( Young et al., 2007 ).

J.S. Young et al.

Neuroscience 148 (1), 10 Aug 2007